Reporter

Part:BBa_E0043:Design

Designed by: Austin Che   Group: iGEM06_MIT   (2008-02-24)


green fluorescent protein (GFP) variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

6 mutations relative to E0040 were inserted. These change the two amino acids at positions 64 and 65 (wild-type GFP numbering): F64L and S65T. An extra silent mutation in the previous amino acid 63 was introduced at the same time to optimize it slightly better for E. coli.


Source

This GFP variant was created via PCR of E0040.

References